The aim of the “BioCasNavi” project is to develop and validate an artificial intelligence (AI)-based platform for the automated design of multi-enzymatic cascade systems for the sustainable synthesis of high-quality, industrially usable compounds from renewable raw materials. BioCasNavi is developing a comprehensive database of enzymatic reactions and conditions as well as AI algorithms for the design and optimization of cascades. The project is accompanied by process development and scaling and socio- and techno-economic studies that define the requirements for sustainable production and responsible use of resources from an industrial perspective. If successful, a new and widely applicable digital platform technology will be developed that enables rapid innovation cycles in the bioeconomy by identifying novel value chains and production processes for sustainable catalysis and synthesis in the bio-based industry.